Identifying an emergent adulterant hydrochlorothiazide in food: A simple lateral flow strip with high sensitivity by time-resolved fluorescence
نویسندگان
چکیده
Hydrochlorothiazide, an antihypertensive and diuretic drug only for clinic purpose, was recently found as adulterant in functional foods. Due to the potential health risk, it is urgent develop a simple rapid assay its identification. In this work, two hapten structures were rationally designed based on molecular modeling, highly sensitive antibody hydrochlorothiazide obtained with 50% inhibitive concentration (IC50) of 6.7 ng/mL first time. A lateral flow strip then developed time resolved fluorescence, demonstrating cut-off value 250 μg/kg capsules tablets, negligible cross-reactivity related compounds. The performance real samples collected from retail markets consistent that confirmed method (LC-MS/MS). As ideal tool, proposed could be applied identify adulterated
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ژورنال
عنوان ژورنال: Food Control
سال: 2023
ISSN: ['0956-7135', '1873-7129']
DOI: https://doi.org/10.1016/j.foodcont.2022.109265